Review



hacat human keratinocytes  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC hacat human keratinocytes
    Effect of x-PVA/HA on <t>HaCaT</t> Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.
    Hacat Human Keratinocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 871 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hacat+keratinocytes/Primary+Epidermal+Keratinocytes%3B+Normal%2C+Human%2C+Adult/pmc13672631-44-11-18
    Average 99 stars, based on 871 article reviews
    hacat human keratinocytes - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Electrospun poly(vinyl alcohol)/hyaluronic acid nanofiber mats for wound care in space: cytocompatibility and effects of UV-C treatment and simulated microgravity"

    Article Title: Electrospun poly(vinyl alcohol)/hyaluronic acid nanofiber mats for wound care in space: cytocompatibility and effects of UV-C treatment and simulated microgravity

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2026.1946755

    Effect of x-PVA/HA on HaCaT Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.
    Figure Legend Snippet: Effect of x-PVA/HA on HaCaT Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.

    Techniques Used: Confocal, Microscopy, Fluorescence, Control, Cell Culture

    Related Articles

    Cell Culture:

    Article Title: Keratinocyte wound healing activity of galactoglycerolipids from the fern Ophioglossum vulgatum L.
    Article Snippet: The genus Ophioglossum consists of ferns with different therapeutic properties, including vulnerary virtues.. The species Ophioglossum vulgatum L. is traditionally used on wounds and burns as an ointment, suggesting the occurrence of lipophilic compounds with tissue repair properties.. We isolated and characterized a galactosyldiacylglycerol mixture (1), composed mainly of 1,2-di-O-linolenoyl-3-O-b-D-galactopyranosyl-glycerol, from the frond dichloromethane extract.

    Article Title: Palbociclib blocks neutrophilic phosphatidylinositol 3-kinase activity to alleviate psoriasiform dermatitis.
    Article Snippet: Department of Medical Research, E-DA Hospital, I-Shou University, Kaohsiung, 824410, Taiwan Graduate Institute of Natural Products, School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, 333324, Taiwan Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, 333324, Taiwan School of Nursing, Fooyin University, Kaohsiung, 831301, Taiwan Research Center for Chinese Herbal Medicine, Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, 333324, Taiwan Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan, 333324, Taiwan Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan, 33305, Taiwan Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City, 24301, Taiwan

    Article Title: Formulative Study and Intracellular Fate Evaluation of Ethosomes and Transethosomes for Vitamin D3 Delivery
    Article Snippet: .. HaCaT keratinocytes (an immortalized human cell line purchased from ATCC ® PCS-200-011TM), fibroblasts (an immortalized human cell line purchased from ATCC ® PCS-201-013TM) and C2C12 myoblasts (an immortalized murine cell line purchased from ECACC 91031101) were cultured in 75 cm 2 plastic flasks using Dulbecco’s modified Eagle medium, supplemented with 10% ( v / v ) FBS, 1% ( w / v ) glutamine, 0.5% ( v / v ) amphotericin B, 100 units/mL of penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and incubated at 37 °C with 5% CO 2 . ..

    Article Title: Flavonoid oligoglycosides from Ophioglossum vulgatum L. having wound healing properties.
    Article Snippet: Two new glycosylated and acylated flavonols, viz. quercetin-3-O-[(6-caffeoyl)-β-glucopyranosyl (1→3) α-rhamnopyranoside]-7-O-α-rhamnopyranoside (2), and kaempferol-3-O-[(6-caffeoyl)-βglucopyranosyl (1→3) α-rhamnopyranoside]-7O-α-rhamnopyranoside (3), together with the known quercetin-3-O-methyl ether (1), were isolated from the aerial parts of the fern Ophioglossum vulgatum L. Their structures were established by means of 1D and 2D NMR spectra, as well as ESI‐MS and ESI-HRMS spectra.. Compounds 1–3 were all found to be active in scratch-wound healing assays on keratinocytes, with 3 being the most active one and showing maximum activity at 20 μM.

    Calcein AM Assay:

    Article Title: Keratinocyte wound healing activity of galactoglycerolipids from the fern Ophioglossum vulgatum L.
    Article Snippet: The genus Ophioglossum consists of ferns with different therapeutic properties, including vulnerary virtues.. The species Ophioglossum vulgatum L. is traditionally used on wounds and burns as an ointment, suggesting the occurrence of lipophilic compounds with tissue repair properties.. We isolated and characterized a galactosyldiacylglycerol mixture (1), composed mainly of 1,2-di-O-linolenoyl-3-O-b-D-galactopyranosyl-glycerol, from the frond dichloromethane extract.

    Article Title: Flavonoid oligoglycosides from Ophioglossum vulgatum L. having wound healing properties.
    Article Snippet: Two new glycosylated and acylated flavonols, viz. quercetin-3-O-[(6-caffeoyl)-β-glucopyranosyl (1→3) α-rhamnopyranoside]-7-O-α-rhamnopyranoside (2), and kaempferol-3-O-[(6-caffeoyl)-βglucopyranosyl (1→3) α-rhamnopyranoside]-7O-α-rhamnopyranoside (3), together with the known quercetin-3-O-methyl ether (1), were isolated from the aerial parts of the fern Ophioglossum vulgatum L. Their structures were established by means of 1D and 2D NMR spectra, as well as ESI‐MS and ESI-HRMS spectra.. Compounds 1–3 were all found to be active in scratch-wound healing assays on keratinocytes, with 3 being the most active one and showing maximum activity at 20 μM.

    In Vitro:

    Article Title: Keratinocyte wound healing activity of galactoglycerolipids from the fern Ophioglossum vulgatum L.
    Article Snippet: The genus Ophioglossum consists of ferns with different therapeutic properties, including vulnerary virtues.. The species Ophioglossum vulgatum L. is traditionally used on wounds and burns as an ointment, suggesting the occurrence of lipophilic compounds with tissue repair properties.. We isolated and characterized a galactosyldiacylglycerol mixture (1), composed mainly of 1,2-di-O-linolenoyl-3-O-b-D-galactopyranosyl-glycerol, from the frond dichloromethane extract.

    Article Title: Flavonoid oligoglycosides from Ophioglossum vulgatum L. having wound healing properties.
    Article Snippet: Two new glycosylated and acylated flavonols, viz. quercetin-3-O-[(6-caffeoyl)-β-glucopyranosyl (1→3) α-rhamnopyranoside]-7-O-α-rhamnopyranoside (2), and kaempferol-3-O-[(6-caffeoyl)-βglucopyranosyl (1→3) α-rhamnopyranoside]-7O-α-rhamnopyranoside (3), together with the known quercetin-3-O-methyl ether (1), were isolated from the aerial parts of the fern Ophioglossum vulgatum L. Their structures were established by means of 1D and 2D NMR spectra, as well as ESI‐MS and ESI-HRMS spectra.. Compounds 1–3 were all found to be active in scratch-wound healing assays on keratinocytes, with 3 being the most active one and showing maximum activity at 20 μM.

    Modification:

    Article Title: Formulative Study and Intracellular Fate Evaluation of Ethosomes and Transethosomes for Vitamin D3 Delivery
    Article Snippet: .. HaCaT keratinocytes (an immortalized human cell line purchased from ATCC ® PCS-200-011TM), fibroblasts (an immortalized human cell line purchased from ATCC ® PCS-201-013TM) and C2C12 myoblasts (an immortalized murine cell line purchased from ECACC 91031101) were cultured in 75 cm 2 plastic flasks using Dulbecco’s modified Eagle medium, supplemented with 10% ( v / v ) FBS, 1% ( w / v ) glutamine, 0.5% ( v / v ) amphotericin B, 100 units/mL of penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and incubated at 37 °C with 5% CO 2 . ..

    Incubation:

    Article Title: Formulative Study and Intracellular Fate Evaluation of Ethosomes and Transethosomes for Vitamin D3 Delivery
    Article Snippet: .. HaCaT keratinocytes (an immortalized human cell line purchased from ATCC ® PCS-200-011TM), fibroblasts (an immortalized human cell line purchased from ATCC ® PCS-201-013TM) and C2C12 myoblasts (an immortalized murine cell line purchased from ECACC 91031101) were cultured in 75 cm 2 plastic flasks using Dulbecco’s modified Eagle medium, supplemented with 10% ( v / v ) FBS, 1% ( w / v ) glutamine, 0.5% ( v / v ) amphotericin B, 100 units/mL of penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and incubated at 37 °C with 5% CO 2 . ..



    Similar Products

    98
    CLS Cell Lines Service GmbH human keratinocytes
    Human Keratinocytes, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hacat+keratinocytes/HaCaT+Cells/pm42884823-307-21-24
    Average 98 stars, based on 1 article reviews
    human keratinocytes - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    CLS Cell Lines Service GmbH human immortalized keratinocyte hacat cells
    Human Immortalized Keratinocyte Hacat Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hacat+keratinocytes/HaCaT+Cells/10__3390_slash_ijms27208973-217-0-5
    Average 98 stars, based on 1 article reviews
    human immortalized keratinocyte hacat cells - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    CLS Cell Lines Service GmbH hacat keratinocyte cell line
    Hacat Keratinocyte Cell Line, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hacat+keratinocytes/HaCaT+Cells/10__1038_slash_s41598___026___74714___x-228-17-21
    Average 98 stars, based on 1 article reviews
    hacat keratinocyte cell line - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    99
    ATCC hacat human keratinocytes
    Effect of x-PVA/HA on <t>HaCaT</t> Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.
    Hacat Human Keratinocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hacat+keratinocytes/Primary+Epidermal+Keratinocytes%3B+Normal%2C+Human%2C+Adult/pmc13672631-44-11-18
    Average 99 stars, based on 1 article reviews
    hacat human keratinocytes - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    98
    CLS Cell Lines Service GmbH human immortalized keratinocytes
    Effect of x-PVA/HA on <t>HaCaT</t> Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.
    Human Immortalized Keratinocytes, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hacat+keratinocytes/HaCaT+Cells/pm42832913-71-24-32
    Average 98 stars, based on 1 article reviews
    human immortalized keratinocytes - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    CLS Cell Lines Service GmbH human keratinocyte cells
    Effect of x-PVA/HA on <t>HaCaT</t> Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.
    Human Keratinocyte Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hacat+keratinocytes/HaCaT+Cells/pmc13629026-97-31-35
    Average 98 stars, based on 1 article reviews
    human keratinocyte cells - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    CLS Cell Lines Service GmbH keratinocytes
    Effect of x-PVA/HA on <t>HaCaT</t> Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.
    Keratinocytes, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hacat+keratinocytes/HaCaT+Cells/10__1038_slash_s41419___026___09252___5-253-7-8
    Average 98 stars, based on 1 article reviews
    keratinocytes - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    CLS Cell Lines Service GmbH human epidermal keratinocytes
    Effect of x-PVA/HA on <t>HaCaT</t> Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.
    Human Epidermal Keratinocytes, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hacat+keratinocytes/HaCaT+Cells/10__1186_slash_s12964___026___03184___5-40-1-7
    Average 98 stars, based on 1 article reviews
    human epidermal keratinocytes - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    CLS Cell Lines Service GmbH human epidermal keratinocyte cell line hacat
    Effect of x-PVA/HA on <t>HaCaT</t> Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.
    Human Epidermal Keratinocyte Cell Line Hacat, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hacat+keratinocytes/HaCaT+Cells/10__1038_slash_s41598___026___69329___1-82-1-7
    Average 98 stars, based on 1 article reviews
    human epidermal keratinocyte cell line hacat - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    Image Search Results


    Effect of x-PVA/HA on HaCaT Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Electrospun poly(vinyl alcohol)/hyaluronic acid nanofiber mats for wound care in space: cytocompatibility and effects of UV-C treatment and simulated microgravity

    doi: 10.3389/fbioe.2026.1946755

    Figure Lengend Snippet: Effect of x-PVA/HA on HaCaT Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.

    Article Snippet: NT2D1 human embryonal carcinoma cells, HEK293 human embryonic kidney cells, and HaCaT human keratinocytes were obtained from the American Type Culture Collection (ATCC, Manassas, VA, United States).

    Techniques: Confocal, Microscopy, Fluorescence, Control, Cell Culture