hacat human keratinocytes (ATCC)
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Hacat Human Keratinocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 871 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 871 article reviews
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Article Title: Electrospun poly(vinyl alcohol)/hyaluronic acid nanofiber mats for wound care in space: cytocompatibility and effects of UV-C treatment and simulated microgravity
Journal: Frontiers in Bioengineering and Biotechnology
doi: 10.3389/fbioe.2026.1946755
Figure Legend Snippet: Effect of x-PVA/HA on HaCaT Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.
Techniques Used: Confocal, Microscopy, Fluorescence, Control, Cell Culture
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